UV- Visible Spectroscopy Fig: UV- Visible Spectrophotometer Content Introduction Instrumentation Working Principle Operating Process Application Introduction: Spectroscopy is the interaction of electromagnetic radiation with matter. When light is absorbed by matter, this results an increase in the energy of the atoms or molecules. Ø In UV-Vis spectroscopy, the UV-Vis light is passed through a sample and the transmittance(T) of light by a sample is measured. Ø Most organic molecules with or without functional groups are transparent in the portions of the electromagnetic spectrum that we call the ultraviolet (UV) and visible (VIS) regions. Ø Wavelength in this region ranges from 200 nm to 800 nm (for UV 200 nm – 400 nm and for Visible region 400 nm-800 nm) Instrumentation: Light source Ø Tungsten filament lamps emit the radiations of 375 nm. Ø The intensity of Hydrogen-De...
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